(= 3)

(= 3). and and = 5). The club chart shows the arithmetic mean of five impartial experiments and their SD. (= 5). Mean values of five impartial B cell contamination experiments are shown. (= 5). After 21 d, the NGFR-positive cells were purified with a specific antibody, and we monitored cell growth for 10 d. Mean values of five impartial B cell contamination experiments are shown. (values were obtained from two-tailed paired assessments performed with the two types of LCLs. (= 5). The figures show cells in metaphase and anaphase. (Scale bar, 10 m.) For each sample, at least 100 mitotic cells were examined. The graphs give the percentage of cells undergoing metaphase or anaphase. Central horizontal lines symbolize means, and error bars show SD. All values were obtained from two-tailed paired assessments performed with the two types of LCLs. (= 5). The bar chart shows the arithmetic mean of five impartial experiments and their SD. (= 5). Mean values of five impartial B cell contamination experiments are given. The M81 EBERs Control M81-Driven B Cell Growth in an Animal Model. We wished to confirm the data gathered in vitro in an in vivo model of EBV contamination and injected resting B cells exposed to M81/E1?+?2 or M81/E1?+?2 Rev into immunodeficient mice (19). Sequential recording of the computer virus weight in the blood by qPCR starting SIS3 4 wk after contamination showed that all mice carried infected SIS3 cells but that this mice infected with the EBER null mutant experienced on average slightly lower blood titers than wild-type counterparts (Fig. 2and = 6 per group). Viral titers in the peripheral blood of infected mice were determined by qPCR at different weeks postinfection. One mouse from your M81/E1?+?2 rev group developed a tumor and died between week 5 and week 6. Central horizontal lines symbolize the median. The results were analyzed with a MannCWhitney test. (test. (for the analysis of total tumor mass. (and and and and = 5). We decided the relative intensity of the signals quantified by the ImageJ software over the loading control (actin). For each sample, the log2-transformed fold switch (FC) is given based on the relative signals displayed by M81/E1?+?2 and M81 wild-type (= 5). Central horizontal lines symbolize means, and error bars show SD. The statistical significance of the assay was evaluated with a one-sample test. (with antibodies specific for cyclin B1 and actin using LCLs generated with M81 or M81/E1?+?2 and synchronized by double thymidine block (= 5). (assessments. (= 2). (= 3). We show one representative example out of three impartial experiments that showed similar results. The graph of bars shows the relative intensity of the signals quantified by the ImageJ software from three impartial samples. The data are given relative to values obtained in main B cells stimulated with CD40L and IL4. Central horizontal lines symbolize means and error bars show SD. (with antibodies specific for AURKA and actin (= 5). (with antibodies specific for AURKB and tubulin (= 6). (with TCL3 antibodies specific for UCHL1 and actin (= 10). The graph of bars shows the relative intensity of the signals quantified by the ImageJ software. We show three representative samples. (= 5). The scatter plot shows the relative intensity of the signals quantified by the ImageJ software from five impartial samples. The data are given relative to values obtained in LCLs generated with M81. Central horizontal lines symbolize means, and error bars show SD. All values were obtained from two-tailed paired assessments performed with the two types of LCLs. ns, not significant. **= 0.0012. (= 2). The graph shows one representative example of UCHL1 expression at multiple days postinfection (dpi). We show one representative example out of two impartial experiments that showed similar results. Open in a separate windows Fig. 4. UCHL1 enhances cyclin B1 and Aurora kinase expression SIS3 in infected cells. (= 3). The same cells were kept in culture and growth was monitored for 10 d. Mean values of three impartial B cell contamination experiments are shown (= 3). (= 4). We decided the relative intensity of the signals quantified by the ImageJ software over the loading control (actin). For each sample, the log2-transformed FC is given based on the relative signals displayed by M81/E1?+?2 and M81 wild-type. Central horizontal lines symbolize means and error bars show SD. The statistical significance of the assay was evaluated with a one-sample test. (= 5). (= 4). For.